Lanthanide binding peptide

Author: mxwb

August undefined, 2024

TīmeklisNative EF-hand sequences bind to calcium or lanthanide ions via oxygen atoms from carboxylic acid side chains (i.e., aspartic acid and glutamic acid), in addition to a carbonyl from the peptide backbone and an outer sphere complex with the ion where a water molecule bridges the ion and amino acid via hydrogen bonding. 22,58 … TīmeklisThis approach has been much more effective with large lanthanide series metals than smaller transition metals. Because chemical conjugation requires additional processing and purification steps and yields a heterogeneous mixture of products, inline incorporation of a peptide tag capable of metal binding is a highly preferable …

Structural Origin of the High Affinity of a Chemically Evolved ...

Tīmeklis2005. gada 21. dec. · A small, versatile and multifaceted co-expression tag would therefore be extremely useful to protein scientists: Lanthanide-Binding Tags (LBTs) fulfill such criteria. These tags are remarkably short peptide sequences (15 to 20 residues) comprising only the encoded amino acids; post-expression addition of a … Tīmeklis2004. gada 12. jūl. · Scientists have begun expressing lanthanide binding tags (LBTs), short peptides with high affinity for REEs, on bacterial cell surfaces to selectively bind trace rare earth metals (Park et... harris county blue form

Design of Lanthanide Fingers: Compact Lanthanide‐Binding ...

Tīmeklis2024. gada 7. febr. · The discovery of bacteria that depend on lanthanides is … Tīmeklis2024. gada 14. apr. · The development of lanthanide-based signalling systems that can monitor the concentration, nature and presence of certain analytes has grown rapidly over the la Tīmeklis2004. gada 7. jūl. · Playing tag with terbium: New hydrophobic contacts and ligating amino acids are revealed in the 2.0-Å resolution X-ray crystal structure of a chemically evolved 17-residue lanthanide-binding peptide ... harris county block book search

Double-lanthanide-binding tags: design, photophysical properties, …

Lanthanide binding peptide

Lanthanide-binding proteins could unlock new sources of valuable …

Tīmeklis2024. gada 13. aug. · The peptide arm connecting the lanthanide binding loop to the C-terminal sequence is, as expected, flexible around the GSG spacer peptide connecting the C-terminal end to the LBT sequence, and does not allow for a complete resolution of the local structure . Interestingly, cryo-EM 3D map reconstruction … Tīmeklis2024. gada 4. aug. · Lanthanide complexes and methods of attaching them to …

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Tīmeklis2003. gada 18. marts · Lanthanide-Binding Affinities. A series of peptide chimeras has been designed based on the structural resemblance of the α−α corners of engrailed and calmodulin proteins ( 12 ). One such peptide, the 33-mer P3W, incorporates the 12-aa consensus EF-hand Ca-binding loop sequence between helices α2 and α3 of … Tīmeklis2024. gada 7. dec. · Brichtová, E.; Hudecová, J.; Vršková, N.; Šebestík, J.; Bouř, P.; …

TīmeklisAbstract. The investigation into the luminescence properties of a lanthanide-binding … Tīmeklis2024. gada 26. maijs · Design concept for lanthanide ion mineralization Our concept uses an artificial peptide that stabilizes an insoluble lanthanide hydroxide complex ( Fig. 1 ). In aqueous solution, metal ions...

Tīmeklis2024. gada 13. apr. · In addition, since the Cu-binding peptide was fused with GFP, the binding between the peptide and Cu decreased the green fluorescence of GFP. Thus, the system could be also used as a biosensor for Cu. 4.1.5 Ni bioremediation. B. subtilis spore surface display has been used to develop protein-coated spores to adsorb Ni … TīmeklisCD spectroscopy showed that the 33-mer peptide P3W folds on binding …

TīmeklisA novel, compact, tunable protein-based lanthanide-binding motif was developed …

Tīmeklis2024. gada 8. okt. · Lanthanide-binding proteins have been examined for the specific lanthanide physical properties and the biological activities of peptide scaffolds. The chirality-induced spin selectivity (CISS) of helical peptide structures is a crucial aspect of spin polarization. Combining CISS-caused spin polarization with a paramagnetic … harris county board of commissionersTīmeklisThe separation of lanthanides from minor actinides such as americium and curium is an important step during the recycling process in the treatment of nuclear waste. However, the similar chemistry and ionic size of lanthanide and actinide ions make the separation challenging. Here, we report that a peptide-based reagent can selectively bind … harris county board meetingTīmeklis2024. gada 10. nov. · A linear peptide consisting of 17 amino acids, named lanthanide binding tag (LBT), shows an interesting recognition pattern for Ln 3+ 11. This peptide originates from a calcium-binding... harris county blank check form charge degree f1Tīmeklis2008. gada 15. febr. · Lanthanide-binding tags (LBTs) are small, genetically encoded, versatile protein fusion partners that selectively bind lanthanide ions with high affinity. The LBT motif features a strategically positioned tryptophan residue that sensitizes Tb 3+ luminescence upon excitation at 280 nm. charge definition physics ks3TīmeklisThe peptide with the strongest binding affinity was the tetra-peptide with alternating … charge deep cycle marine batteryTīmeklis2003. gada 4. apr. · Lanthanide-binding tags (LBTs) are expressible, multitasking … charged eldritch wand caps