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Cy5 filter

WebA specific region in Gardnerella vaginalis genome is amplified and fluorescence detection is accomplished using the Cy5 filter. An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with the Texas RED filter. WebEmission filter: 585/16 nm: 620/15 nm: 695/40 nm: 780/60 nm: Channel: YL1: YL2: YL3: YL4: Compatible fluorescent proteins: mOrange RFP dTomato: mCherry DsRed mKate mStrawberry : Antibody labels: Alexa Fluor 568 PE Qdot 565 Qdot 585: Alexa Fluor 594 PE- Alexa Fluor 610 PE-Texas Red: PE-Alexa Fluor 700 PE-Cy5.5 Qdot 705

Lasers and Dyes for Multicolor Flow Cytometry - BD Biosciences

WebPerCP-Cy5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye … WebThe scanners actually use diverse laser emission wavelengths (typically 532 nm and 635 nm) and filter wavelengths (550-600 nm and 655-695 nm) to avoid background contamination. They are thus able to easily distinguish colors from Cy3 and from Cy5, and also able to quantify the amount of Cy3 and Cy5 labeling in one sample (multiparametric ... pa line waveforms https://cgreentree.com

Red Excitation Filter Sets Nikon’s MicroscopyU

WebThe scanners actually use diverse laser emission wavelengths (typically 532 nm and 635 nm) and filter wavelengths (550-600 nm and 655-695 nm) to avoid background … Web49006 - ET - Cy5. Recommended to detect CL-650 in Cell IDx Four Color assay. Add some data! Use the legend to add fluorochromes, filters sets and individual filters to the plot. … WebApr 11, 2024 · The microfluidic device is imaged with a standard flatbed imager, which is equipped with combinations of lasers and filters for the four fluorophores, FAM, HEX, Texas Red and Cy5. Last, the resulting images are analyzed using our customized software program to evaluate the intensity of the fluorescence generated from the four TaqMan … palin family cottage hotel บ้านและสวน

Optical Filter Sets for Fluorescence Applications - Chroma

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Cy5 filter

Cy3 Filter Set for Fluorescence Spectroscopy - Elliot Scientific

WebApr 11, 2024 · Sulfo-Cyanine7 TSA磺化氰化物7是光谱近红外部分的水溶性荧光团,广义的Cy染料是一种结构和应用非常广泛的一大类荧光化合物。. 在这里我们主要讨论用于生物标记的Cy染料,包括Cy3,Cy3.5,Cy5,Cy5.5,Cy7和Cy7.5。. 所有的这些染料都是上世纪70年从染料ICG(Indocyanine ... WebCommonly used filter sets & relevant fluorescent proteins. Commonly used filter sets for multicolor imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed for DAPI / Fluorescein / Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / 561 / 640 nm). In our hands the ...

Cy5 filter

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WebMar 15, 2024 · The high efficiency filter sets (HE) are characterized by particularly steep edges and exceptionally high transmission values. For the same signal intensity, the … WebApr 10, 2024 · Then Cy5-LNP/Cy5-cLNP was added at a concentration of 1 mg/ml and incubated for 1 h with gentle shaking at room temperature. After incubation, the plates were washed with 1× PBS for three times. ... The pellet was resuspended in 500 μl of PBS and passed through a FACS filter (Partec, UK) into a FACS cartridge (Partec, UK). The …

WebFluorescense detection is accomplished using the FAM, HEX, Texas Red and Cy5 Filters. In the first PCR tube with PCR Master Mix 1, Herpes Simplex Virus 1 genome is amplified and fluorescence detection is accomplished using the FAM filter, Herpes Simplex Virus 2 genome is amplified and fluorescence detection is accomplished using the Cy5 filter ... WebAvailable filter pairs for IVIS Instrumentation Filter recommendations for common fluorescent proteins, dyes and Quantum Dots using ... Cy5.5 675nm 720nm Excitation Emission ICG 745nm 840nm Excitation Emission Cy7 745nm 800nm Excitation Emission PKH26 535nm 580nm 0 20 40 60 80 100 400 500 600 700 800 0 20 40 60 80 100

WebCy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the … WebThe Cy5 fluorophore is also the basis for the lipophilic neuronal and long-term DiD cell tracing reagents, which append alkyl tails (≥12 carbons) onto the core fluorophore. For …

WebCy5 Filter Set . This Iridian single band filter set consists of the 628-40 EX, 692-40 EM and 660 DM filters. It is optimised for the cyanine dye Cy5. …

WebThe AriaMx Real-Time PCR system is a fully integrated qPCR solution for amplification, detection, and data analysis, combining a novel thermal cycler, an advanced optical system with LED excitation source, and comprehensive data analysis software. AriaMx has a closed-tube PCR detection format and can be used with many fluorescence detection ... pal infocom technologies pvt.ltdWebCy3 Filter Set. This Iridian single band filter set consists of the 531-40 EX, 593-40 EM and 562 DM filters. It is is optimised for the cyanine dye Cy3, but is also suitable for use with DsRed, Alexa Fluor 555, Calcium Orange, LysoTracker Yellow, MitoTracker Orange, Phycoerythrin, Rhodamine Phalloidin and 5-TAMRA. summit urology american forkWebCy5.5 Filter Set. This Iridian single band filter set consists of the 655-40 EX, 716-60 EM and 685 DM filters It is optimised for the cyanine dye Cy5.5 and similar fluorophores like Alexa Fluor 680 and 697. The set contains excitation, emission and dichroic filters designed to fit in standard filter cubes from the leading microscope and third ... palin familyWebCy5.5 Filter Set. This Iridian single band filter set consists of the 655-40 EX, 716-60 EM and 685 DM filters It is optimised for the cyanine dye Cy5.5 and similar fluorophores like … summit urology marltonWebOptical Filters. For sensitive imaging at the desired resolution on our cellular, tissue, and in vivo imaging systems, we offer custom-designed optics, including optical filters and filter cubes. Products & Services ( 15 ) summit urogynecology akronWeb20 rows · Alexa Fluor dyes—Emission spectra. Figure 1. Emission … summit ut county assessorWebThe Aria Real-Time PCR System is a fully integrated quantitative PCR amplification, detection, and data analysis system. The system combines a state-of-the-art thermal cycler, an advanced optical system with an LED excitation source, and complete data analysis software. The Aria System can be used with many fluorescence detection chemistries … summit user name