Biotinylated mouse tim4-fc

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August undefined, 2024

WebOutline: This product is for research use only. Do not administer it to human. This is a recombinant fusion protein; the extracellular domain of mouse TIM4 (TIMD4) is linked to the N-terminus of human IgG 1 Fc domain … WebRecombinant Mouse TIM-4 (Catalog # 2826-TI) inhibits anti-CD3-induced proliferation of stimulated human T cells. Human T cells cultured for 72 hours with PHA are incubated …

Anti-TIMD4 / TIM4 / TIM-4 Antibody Hamster-Armenian anti-Mouse …

WebThe cells were then stained with PerCP-Cy™5.5 Rat Anti-Mouse CD11b (Cat. No. 550993/561114) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Panel) or PE Rat Anti-Mouse TIM-4 antibody (Right Panel). Two-color flow cytometric contour plots showing the correlated expression patterns of TIM-4 (or Ig Isotype control staining ... WebMar 27, 2005 · The newly identified TIM family of proteins is associated with regulation of T helper type 1 (TH1) and TH2 immune responses. TIM-1 is genetically linked to asthma and is a receptor for hepatitis A ... philly to lansing michigan

The phosphatidylserine receptor TIM4 utilizes integrins as …

WebTIM-4 (T cell; immunoglobulin; Mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIM-4 is exclusively expressed on antigen-presenting cells, where it mediates phagocytosis of apoptotic cells and plays an important role in maintaining tolerance. TIM-4 binds specifically to TIM-1 which is also ... WebTo produce an mAb against Tim4, Armenian hamsters were immunized with Tim4-Fc, in which the extracellular region of mouse Tim4 was joined to human IgG Fc region (11). Lymphocytes from the immunized hamsters were fused with a mouse myeloma NSObcl2 (17). One hybridoma (Mat4-9-5) that was suitable for immunohistochemical staining, WebSpecificity / Sensitivity. Anti-mouse IgG (H+L), (Biotinylated) reacts with the heavy and light chains of most mouse immunoglobulins. No cross-reactivity to other serum proteins … philly to las vegas flight time

Anti-Mouse Secondary Antibodies - Jackson Immuno

PE Rat Anti-Mouse TIM-4 - BD Biosciences

WebFeb 9, 2024 · Needle-like multi-walled carbon nanotubes (MWCNTs) are engulfed by macrophages, which cause inflammation and asbestosis-like pathogenesis in rodents; however, how macrophages recognize … WebTim-4 can isolate extracellular vesicles from different species (human, mouse, rat, etc.) containing phosphatidylserine (PS) at their surface. Biological Activity Measured by its … philly to lansdale paWebTim4 alone did not support efferocytosis, but enhanced TAM-dependent efferocytosis. Resident peritoneal macrophages, Kupffer cells, and CD169+ skin macrophages … philly to lexington

"WebApr 1, 2024 · Exosome concentration by ultracentrifugation and Tim4 affinity columns We also concentrated Exo by the Tim4-affinity method using a MagCapture Exosome Isolation Kit PS (Wako, Japan). Streptavidin magnetic beads (0.6 mg), bound with 1 μg of biotinylated mouse Tim4-Fc, were added to precleared CSs supplemented with 2 mM … " - Biotinylated mouse tim4-fc

Biotinylated mouse tim4-fc

TIM4-Affinity Methods Targeting Phosphatidylserine for Isolation …

WebNov 10, 2024 · Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag (MALS & BLI verified) FCM-M82W5: HEK293: Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, His, Avitag™ (MALS & BLI verified) FCM-M82W6 ... WebMouse Ig cocktail. Formulation. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation. The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. Concentration. 0.5 mg/ml. Storage & Handling. The antibody solution should be stored undiluted between 2°C and 8°C.

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WebSep 23, 2016 · Nevertheless, Tim4-Fc-conjugated beads were still able to isolate sEVs efficiently from 10K sup of pMac which abundantly express … WebTIM4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by …

WebRegarding Tim4 expression, cells were stained with PE-labeled cIg, or RMT4-54 (anti-Tim4: BioLegend). Mouse peritoneal resident cells were pretreated with clone 93 (anti-CD16/32: BioLegend) to block Fc receptors, and then stained with FITC-anti-CD11b (BioLegend) and the above mAbs. These cells were analyzed by an Accuri C6 cytometer (BD ... Webadjective. bio· tin· y· lat· ed ˌbī-ə-ˈti-nə-ˌlā-təd. : combined with biotin. The PCR reaction uses biotinylated primers to define a sequence of approximately 207 nucleotides within …

WebAug 31, 2024 · The EVs were isolated from the sera of PC patients or NC in cohort-1 using the Magcapture Exosome Isolation Kit (Fujifilm) according to the manufacturer’s protocol. In brief, 0.6 mg of streptavidin magnetic beads, bound with 1 µg of biotinylated mouse Tim4-Fc, was added to the serum supplement and rotated overnight at 4 °C. WebMay 19, 2024 · 2.3 TIM4-Affinity Beads. 1. Mouse TIM4-Fc protein. 2. Maleimide-PEG11-Biotin: 25 mM Maleimide-PEG11-Biotin in dimethylsulfoxide (DMSO). To prepare 250 …

WebTIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich …

WebMeaning of biotinylated. What does biotinylated mean? Information and translations of biotinylated in the most comprehensive dictionary definitions resource on the web. philly to leesport paWebFUJIFILM biotinylated mouse tim4 fc Biotinylated Mouse Tim4 Fc, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed … philly to lancaster busWebMouse TIM-4 Antibody Summary Species Reactivity Mouse Specificity Detects mouse TIM-4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with … philly to lehighWebMar 15, 2024 · Then concentrated CM were incubated with Streptavidin Magnetic Beads (60 mg) accompanied by additional 1 μg of biotinylated mouse Tim4-Fc, 350 μL Exosome Capture Immobilizing Buffer, and 50 μL Exosome Binding Enhancer overnight at 4 °C. The next day, the beads were washed with washing buffer for three times and the bound EVs … philly to las vegasWebFeb 9, 2024 · Tim4 is involved in recognition of MWCNTs by mouse peritoneal macrophages. (A) Recognition of MWCNTs (30 μg/mL) by WT or Tim4−/− mouse … tsc hearingsWebMouse TIM4-Ig fusion protein Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide ... FC - Quality tested. Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per ... philly to lancaster driveWebThe TIM4-affinity isolation method helps overcome the disadvantages of the affinity isolation method and enables the isolation of heterogeneous SEVs at high purity. This … tsc hearing type